Punisher #4 (Civil War)

A large group of villains gather to mourn the death of Stilt-Man (Wilbur Day). After a brief appearance by Spider-Man (Peter Parker) the villains decide to ramp up their villainy, but before they can even leave, a bomb set up by the bartender (a disguised Punisher (Frank Castle)) blows up the bar, presumably killing many of them.

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